Lucigen’s E
Lucigen offers the following choices of strain: 1) E. cloni 10G Competent Cells: Routine cloning, subcloning, and plasmid isolation with or without blue/white screening. 2) E. cloni 10GF′ Competent Cells: Contain the F′ plasmid for infection with M13 to produce ssDNA.
And the following choices of efficiency: 1) E. cloni 10G Chemically Competent Cells (> 1 × 10^9 cfu/µg pUC DNA; (> 1 × 10^8 in 96-well plates). Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs). Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput. 2) E. cloni 10GF´ Chemically Competent Cells (≥5 × 10^8 cfu/µg pUC DNA). An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc. 3) E. cloni 10G Chemically Competent Cells, Subcloning Grade (≥1 × 10^6 cfu/µg pUC DNA). The best value available anywhere for simple cloning and plasmid propagation.
cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use-only common laboratory equipment is required. These cells provide solutions for a wide range of applications at unparalled economical prices.
Información para pedidos: Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
