Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

The Protein Extraction & Lysis Buffer (PE LB™) systems ensure good protein recovery, while maintaining the biological activity of the proteins. The solubilized proteins are suitable for enzyme assays (see figure), electrophoresis, folding studies, chromatographic studies and many other downstream applications.

The PE LB™ systems offer a wide selection of buffers for lysis and extraction of proteins from bacteria, yeast, animal cells and tissues. The PE LB™ systems are based on a proprietary combination of organic buffering agents, mild non-ionic detergents, and a combination of various salts to enhance extraction of proteins and maintain stability of biological activities of the proteins.

Depending on the application, additional agents such as chelating agents, reducing agents and protease and phosphatase inhibitors may be added to the PE LB™ buffer system.

The PE LB™ systems are compatible with most downstream applications including enzyme assays, ELISA, chromatographic applications, gel electrophoresis, western blotting and protein folding procedures.

The correct selection of lysis and extraction buffers requires knowledge of the proteins of interest and the stability of their biological activities