Kits provide a fast and economical method for the purification of total DNA (including genomic, mitochondrial, and chloroplast DNA) from plant tissue and cells

• Sample Size: up to 100mg fresh plant tissue/25mg dry plant tissue
• Expectant Yield: up to 50µg DNA
• Operation Time: 60min or less
• Elution Volume: 50-200µl
• Binding Capacity: 50µg

The Genomic DNA Mini Kit for Plants and Fungi provide a fast and economical method to isolate total DNA (genomic DNA, mitrochondrial, and chloroplast) from plant tissue and cells. Samples are disrupted both by grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix of the spin column. The contaminants are washed with an ethanol based wash buffer. The genomic DNA is then eluted by a low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. This entire procedure can be completed in 30 minutes or less and the purified genomic DNA is ready for PCR, Real-Time PCR, Southern Blotting, or RFLP.