The NEBNext Immune sequencing kit (human) enables exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains.
- Enrich for and sequence both B cell receptors (BCR) and T cell receptors (TCR)
- Generate full-length immune gene repertoires of B and T cells
- Accurately quantify transcripts with unique molecular identifiers (UMIs)
- Analyze data using an open-source Galaxy workflow
This includes modular primer sets, providing information for complete V, D, and J segments and full isotype information analysis (IgM, IgD, IgG, IgA and IgE), and TCR (alpha symbol) and TCR (beta symbol) chain characterization. A unique, UMI-based mRNA barcoding process allows PCR copies derived from an individual molecule to be converted to a consensus sequence. This improves sequence accuracy and eliminates PCR bias, allowing for quantitative digital molecule counting. Immune repertoire sequencing is frequently used to analyze immune responses, both current and distant. Areas of particular interest include characterization of autoimmune diseases, oncology, and the discovery of neutralizing antibodies against infectious disease.
