DNA Ligase T4Supplier: Rockland Immunochemicals
10801-156EA 266.44 USD10801-156 10801-154
DNA Ligase T4
Nucleic Acid Reagents Cloning Kits and Ligation
DNA Ligase T4 Molecular Biology Grade
- 50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH 7.5 @ 25°C.
- Ligation of nicks in dsDNA and joining dsDNA fragments with complementary overhangs > 2 bases in length.
Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids.
One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37° C (Weiss, B., et al., JBC 243, 4543 (1968).