The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.
- Magnetic Purification of Polyhistidine- or HQ-tagged Proteins from E. coli or Mammalian Cell Lysates
- No centrifugation or vacuum required after cell lysis
- Particles are compatible with a variety of common buffers
- Binding capacity up to 1mg of polyhistidine-tagged protein per 1ml of particles
The MagneHis Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidine- or HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek 2000 or FX or Tecan Genesis(R) RSP.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Environmentally Preferable:
- SizeSupplier no.
- MagneHis Ni-Particles, 10ml
- MagneHis Ni-Particles, 2ml