Generate highly complex, Illumina-compatible DNA fragment libraries with ≥50 pg of DNA with this quick & easy kit
- Produces complex libraries with improved sequencing depth uniformity and coverage
- Requires only 50 pg to 75 ng input of sheared/fragmented DNA
- Minimizes PCR bias to improve coverage uniformity
- Saves time with a fast, 3 hour protocol
The NxSeq® UltraLow DNA Library Kit combined with a NxSeq® Single Indexing Kit enable the construction of high quality DNA fragment libraries with minimal DNA inputs – as low as 50 pg depending on sample type and Illumina sequencer used
To enable the use of such low input amounts while still generating excellent sequencing results, we optimized each step of the protocol to ensure peak performance on Illumina sequencers. To help with the initial steps of DNA fragmentation, we’ve included guidance for mechanical shearing on an instrument like a Covaris LE220 and an optimized protocol for enzymatic fragmentation using dsDNA Shearase Plus from Zymo Research. Not only will you produce high quality libraries using this kit, but once you generate the fragmented DNA, library prep is quick and easy - about 3 hours.
This system is extensively tested with whole genome sequencing (WGS) and resequencing applications for de novo sequencing or SNV/mutation or copy number variation (CNV) identification. The system can, however, be used in other NGS applications which require PCR-amplified libraries such as ChIP-seq, exome-seq, or with challenging sample types such as metagenomic and FFPE DNA samples.
To build sequenceable libraries with the NxSeq UltraLow DNA Library Kit, you must purchase at least one NxSeq Single Indexing Kit. Libraries made with these kits are only compatible with Illumina sequencing instruments (e.g. NextSeq, MiSeq and the HiSeq 2500, 3000, 4000, X Ten, and X Five).
Información para pedidos: The NxSeq UltraLow DNA Library Kit, 12 Reactions contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG), 2X PCR Master Mix (MM) and Elution Buffer (EB). At least one NxSeq Single Indexing Kit is required to complete library prep and must be purchased separately.
Each NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. Each NxSeq® Single Index equals the TruSeq® LT Index with the same number.
Precaución: For research use only. Not for human or diagnostic use.