Specifically dephosphorylate 5´-triphosphorylated RNA, such as primary RNA transcripts, but not 5´-capped mRNA
- Specific: Removes the γ and β phosphates from 5´-triphosphorylated and the β phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5´-capped RNA
- Heat Inactivated: Heat at 65°C for 20 minutes to kill enzyme activity
- Flexible: Use for various applications such as 5´-RNA ligation-tagging using T4 RNA Ligase, analysis of 5´-end structure of RNA, and preparation of substrate RNAs for subsequent degradation with Terminator™ 5´-Phosphate-Dependent Exonuclease
RNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists
RNAs with a 5´-diphosphorylated end are also converted to 5´-monophosphorylated RNA by RNA 5´ Polyphosphatase:
5´ ppN—OH 3´ to 5´ pN—OH 3´ + Pi
RNA Polyphosphatase has no activity on RNA with a 5´ cap (e.g., 5´ m7GpppN—OH 3´), or a 5´-monophosphorylated end (5´ pN—OH 3´). However, both NTPs and dNTPs are substrates for the enzyme, yielding the corresponding NMPs and dNMPs + inorganic phosphate:
(d)NTP to (d)NMP + 2Pi
Applications include:
Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase
Analysis of 5´-end structure of RNA
Preparation of substrate RNA molecules for subsequent degradation using the Epicentre Terminator™ 5´-Phosphate-Dependent Exonuclease
Certificaciones: For research use only. Not for human or diagnostic use.
Información para pedidos: Contents include enzyme and a 10X reaction buffer.
