T4 RNA Ligase, Promega

Supplier: Promega
M1051
PAM1051EA 172.03 USD
PAM1051
T4 RNA Ligase, Promega
Nucleic Acid Reagents Cloning Kits and Ligation
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

  • Joins ssRNA or ssDNA onto the 5´-Phosphoryl End of ssRNA or ssDNA
  • Adds [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA
  • Can be inactivated by heating at 65°C for 15 minutes
  • Supplied at a concentration of 10 u/microl
  • For ligation, Labeling and cloning

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes the addition of [5'-32P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.

Precaución: Storage conditions –30...–10 °C
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