ePaTOX II, Analytik Jena
Supplier: Analytik Jena
847-00101-2
CA10053-356
ePaTOX II, Analytik Jena
The ePaTOX II is a versatile instrument for highly sensitive, chip-based detection of proteins, toxins, nucleic acids and other biomolecules in a wide range of samples.
- Identification and quantification of toxins and pathogens
- Fully automated, stable, electrochemical detection on microarrays
- Plug & play operation using disposable one way chipsticks
- Robust housing for mobile NBC detection systems such as those found in emergency management vehicles or for stationary monitoring systems used in public buildings
- In-house production of monoclonal antibodies for quantification (BoNT-A, -B, -F, SEB, ricin) and detection (BoNT-F, SEB and ricin)
- Short measurement times (approx. 20 minutes for toxins/ proteins; approx. 10 minutes for DNA/RNA)
- Limit of detection = approx. 0.5 ng/mL
The ePaTOX II is a versatile instrument for highly sensitive, chip-based detection of proteins, toxins, nucleic acids and other biomolecules in a wide range of samples
The principle of chip detection with the ePaTOX II is based on an electrochemical reaction. The process utilizes chips with extremely fine, nanoscale electrode structures produced using state of the art silicon semiconductor technology. A biochip of this kind includes 16 measuring positions which are then loaded with various receptor molecules specific to the application. Depending on how the biochip is configured, this allows scientists to detect multiple toxins or pathogens in parallel on a single chip. The sample to be studied is automatically pumped across the chip during the detection process, allowing the target molecules in the sample to bind to their complementary receptor molecules (e.g., antibodies, oligonucleotides), which are immobilized on the surface of the chip. This step is followed by enzyme marking. Fixed on the chip in this way, the enzyme then converts a substrate that can be detected in an electrochemical reaction. The electrode structures on the chip make it possible to measure an electrical signal; the size of the signal is directly proportional to the concentration of the target molecules in the sample. This electrochemical detection principle makes it possible to achieve high analytical sensitivity, and the resulting detection system is immune to the effects of turbidity and other sources of optical interference.
The ePaTOX II is particularly easy to use: its fully automated, user-friendly software controls the analytical process, provides the user with necessary instructions, and analyzes the test results. The system also displays important notes for error-free routines. A simple syringe filtration step is used for preparing protein or toxin samples. Ready-to-use kits are also available.
Simultaneous analysis of multiple electrode positions is the test principle underlying an array-based, electrical biochip. For detection to occur, the target molecules must bind to the specific receptor molecules immobilized on the chip. To this end, each of the 16 measuring positions have been modified with corresponding receptor oligonucleotides and/or antibodies, thereby enabling detection of multiple biomolecules in parallel within a single sample. Because an exceptionally wide variety of receptor molecules can be immobilized on the chip, a considerable number of solid-phase-bound detection systems (such as ELISA, or enzyme-linked immunosorbent assay) can be transferred to the biochip. The ePaTOX II is ideal for integration into mobile ABC systems and for monitoring systems used in public buildings such as airports, harbors, and subways or laboratories. The system offers extensive, group-specific detection options for toxins and/or pathogens. Toxins detected include ricin, staphylococcus enterotoxin B and botulinum toxin A, B and E. The system can detect the following pathogens after appropriate DNA extraction and PCR: Bacillus anthracis, Yersinia pestis, Francisella tularensis and Orthopoxvirus. One special feature of the system is its high tolerance to a variety of different sample matrices (such as water, milk, starch, flour, juice, soil, aerosols, etc.)
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