Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific

Supplier: Thermo Fisher Scientific
Pierce
20343 20343
PI20343EA 356.68 USD
PI20343 CAPI20343
Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific
Protein Extraction Protein Extraction Reagents
Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

  • Eliminates autolysis and contamination of a sample with the protease
  • Allows control of the digestion by removing the pepsin
  • Pepsin is used in the laboratory analysis to generate antibody fragments
  • Immobilized pepsin is more stable against heat-induced denaturation, resulting in longer maintenance of activity

Pepsin has broad substrate specificity. It cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. Pepsin is used to generate F(ab')2 fragments by removing only that portion of the Fc domain beyond the hinge region. The resulting F(ab')2 fragments are composed of two antibody-binding Fab' fragments connected by disulfides in the hinge region.

Precaución: For Research Use Only. Not for use in diagnostic procedures.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR