NEBNext Multiplex Oligos for Illumina (96 Index Primers), New England Biolabs

Supplier: New England Biolabs
E6609L E6609S E6609S E6609L
103218-908EA 2704.97 USD
CA103218-910 103218-908 CA103218-908 103218-910
NEBNext Multiplex Oligos for Illumina (96 Index Primers), New England Biolabs
Nucleic Acid Reagents Primers and Probes
Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Adaptors enable high-efficiency adaptor ligation and high library yields, with minimized adaptor-dimer formation.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Convenient 96-well plate format with pierceable foil seal
  • Includes USER Enzyme

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER Enzyme (a combination of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal. NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
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