TurboCells® Chemically Competent E.coli are specially optimized to achieve excellent transformation efficiency with a novel 3-minute transformation protocol.
- Fast and easy protocol
- Ideal for high-throughput transformations and routine cloning experiments
- Allow efficient transformation without the need for a lengthy transformation reaction
The genotype of TurboCells is suitable for most cloning needs such as blue/white color selection, generation of plasmid vector based libraries, and the ability to efficiently transform large plasmids
When using the 3-minute transformation protocol, transformation efficiencies of up to 5 x 107 to 1 x 108 cfu/µg of supercoiled DNA can be achieved, which is more than sufficient for most cloning experiments. When using the traditional 1.5 to 2 hour transformation protocol, efficiencies of 1 x 109 cfu/µg or greater can be achieved.
TurboCells F' is also available for use with M13 cloning vectors.
Información de suministro: Includes TurboCells® chemically competent E.coli, TurboCells® transformation buffer (5 mL) and pUC19 positive control plasmid (20 µL, 10pg/µL).