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VWR® Salt Activated Nuclease

Supplier: Avantor
E. coli were lysed &treated for 20 minutes at room temperature with (l) or without (r) SAN. The SAN treated lysate became less viscous than the untreated sample, and when both were centrifuged briefly, only the SAN treated sample formed a pellet. The untreated lysate was too viscous to pellet
E. coli lysates containing 75 µg/ml DNA were resolved by agarose gel electrophoresis after 30-minute incubations at 2°C or 37°C with increasing units of SAN.SAN activity was terminated by addition of EDTA. At both temperatures, SAN degraded the DNA into smaller fragments with increasing units of SAN
VWR® Salt Activated Nuclease
VWR® Salt Activated Nuclease
E. coli were lysed &treated for 20 minutes at room temperature with (l) or without (r) SAN. The SAN treated lysate became less viscous than the untreated sample, and when both were centrifuged briefly, only the SAN treated sample formed a pellet. The untreated lysate was too viscous to pellet
E. coli lysates containing 75 µg/ml DNA were resolved by agarose gel electrophoresis after 30-minute incubations at 2°C or 37°C with increasing units of SAN.SAN activity was terminated by addition of EDTA. At both temperatures, SAN degraded the DNA into smaller fragments with increasing units of SAN
VWR® Salt Activated Nuclease
VWR® Salt Activated Nuclease
VWRV1B1664-5KU
10147-170EA 406.89 USD
10147-170
VWR® Salt Activated Nuclease
Enzymes
VWR’s Salt Active Nuclease (SAN) is a very active non-specific endonuclease from Pichia pastoris that cleaves double- and single-stranded DNA and RNA

  • Store frozen (-20 – 0°C)
  • Stable 6 months stored cold (2 – 8°C) and two years frozen (-20 – 0°C)
  • SAN tolerates multiple freeze-thaws

It is active at above neutral pH (optimal pH 8.5) over a wide temperature range (10- 40°C), and unlike other nucleases it has optimum activity at high concentrations of salt (0.5M NaCl). SAN is ideal for use in removal of DNA and RNA from cell extracts and protein samples. Thus, it is very useful in recombinant protein purifications.
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