Q5® Site-Directed Mutagenesis Kit, New England BiolabsSupplier: New England Biolabs
102855-188EA 220.37 USD102855-188 CA102855-188
Q5® Site-Directed Mutagenesis Kit, New England Biolabs
Nucleic Acid Reagents Nucleic Acid Modification and Labelling
The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours
The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency NEB 5-alpha Competent E. coli, provided with the kit, ensures robust results with plasmids up to at least 20 kb in length.