Q5® Site-Directed Mutagenesis Kit (Without Competent Cells), New England BiolabsSupplier: New England Biolabs
102877-562EA 145.56 USDCA102877-562 102877-562
Q5® Site-Directed Mutagenesis Kit (Without Competent Cells), New England Biolabs
Nucleic Acid Reagents Nucleic Acid Modification and Labelling
The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours
The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, not supplied, ensures robust results with plasmids up to at least 20 kb in length.