Dissolve 10 mg hyaluronic acid in 25 mL 0.1 M sodium phosphate buffer: pH 5.3 with 0.15 M sodium chloride.
Amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37°C and pH 4.0
Inhibitors: Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+, Stabilizers: Sodium chloride acts as a stabilizer.
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