gPURE Cultured Cell Extraction Reagent Kit, IBI ScientificSupplier: IBI Scientific
IB47431 IB47432 IB47430
89493-600EA 193.74 USD89493-600 89493-602 89493-598
gPURE Cultured Cell Extraction Reagent Kit, IBI Scientific
Protein Reagents Protein Extraction Reagents
IBI gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA
Initially, cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed, DNA is precipitated and rehydrated. The high-quality extracted DNA is ready for use in a variety of downstream applications or archiving.
Each kit contains Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0), and RNase A (10 mg/mL). The Instruction Manual contains protocols for 3-5 x 106 and 3-5 x 107 adherent and suspension cultured cell procedures.
This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes and provides a convenient sample-storage procedure with minimal hands on time.