Luciferin is a substrate for the enzyme firefly luciferase
D-Luciferin, potassium salt is the more commonly water soluble substrate for cell-based and in vivo reporter assays. D-luciferin, sodium salt is an alternative salt form with higher water solubility. D-luciferin, free acid is soluble in methanol or DMSO, but only sparingly soluble in water. DMNPE-caged D-luciferin is a D-luciferin ester derivative which can cross cell membranes efficiently. Once inside the cells, the ester is continuously hydrolyzed to a supply of D-luciferin. Alternatively a burst of D-luciferin release can be generated by UV photolysis.
Biotium also offers Firefly and Renilla Luciferase Assay Kits, and ATP-Glo Bioluminescent Cell Quantitation Kit.
Luciferase is encoded by the luc gene, which is widely used as a reporter gene. Luminescence at 560 nm is generated when luciferase catalyzes the oxidation of luciferin by an ATP-dependent process. Cells and tissues have very low intrinsic bioiluminescence, allowing for highly sensitive detection of lucifease expression. In addition, luciferin/luciferase can be used for highly sensitive measurements of ATP levels.
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- D-luciferin, potassium saltD-luciferin, potassium salt1010110 mg10101Each (10mg)$102.23
- D-Luciferin, potassium saltD-Luciferin, potassium salt89138-27810101-150 mg10101-1Each (50mg)$259.81
- D-Luciferin, potassium saltD-Luciferin, potassium salt89138-28010101-21 g10101-2Each (1g)$1,088.37
- D-Luciferin, sodium saltD-Luciferin, sodium salt89138-2821010210 mg10102Each (10mg)$101.14
- D-Luciferin, sodium saltD-Luciferin, sodium salt89138-28410102-150 mg10102-1Each (50mg)$272.83
- D-Luciferin, sodium saltD-Luciferin, sodium salt89138-28610102-21 g10102-2Each (1g)$854.08
- D-Luciferin, free acidD-Luciferin, free acid89138-2701010010 mg10100Each (10mg)$80.55
- D-Luciferin, free acidD-Luciferin, free acid89138-27210100-150 mg10100-1Each (50mg)$234.44
- D-Luciferin, free acidD-Luciferin, free acid89138-27410100-21 g10100-2Each (1g)$842.71
- DMNPE-caged D-LuciferinDMNPE-caged D-Luciferin89138-288101035 mg10103Each (5mg)$132.56