T3 DNA Ligase, New England Biolabs

Supplier: New England Biolabs
M0317L M0317S M0317S M0317L
102500-168EA 73.8 USD
102500-168 102500-170 CA102500-170 CA102500-168
T3 DNA Ligase, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

It will catalyze the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.
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