T4 DNA Ligase, Cytiva

Supplier: Cytiva
27-0361-01 27-0361-01
95040-312EA 1025.92 USD
CA95040-312L 95040-312
T4 DNA Ligase, Cytiva
Nucleic Acid Reagents Cloning Kits and Ligation
T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

  • For covalently linking DNA fragments in as little as 30 minutes
  • Single dose, ambient-temperature-stable reaction mixes

Catalyzes the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol. Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 µl, yields a solution of reaction buffer, T4 DNA ligase, and ATP.

Storage conditions: Room Temperature

Información de suministro: Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 µl, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
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