This kit is designed for running the first dimension of 2-D electrophoresis using precast Immobiline DryStrip gels, see Immobiline DryStrip Gels. You can apply up to 100 µL of sample per strip and run up to 12 strips.
- For use of immobiline DryStrip gels with Multiphor II electrophoresis system
The instruction manual presents all necessary information for the entire method for horizontal 2-D electrophoresis. By appropriate adaptations, the SDS electrophoresis can also be performed using vertical electrophoresis equipment. Isoelectric focusing with an immobilized pH gradient (IPG), makes true isoelectric focusing possible and significantly improves the reproducibility of the spot distribution along the pH gradient axis of 2-D maps. IPG also makes it possible to focus basic proteins in the gel and to obtain distinct protein spots. Immobiline DryStrip kit facilitates sample application and running and equilibration of Immobiline DryStrip for the first dimension in 2-D (two-dimensional) electrophoresis.