FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

BML-KI105-0300
89160-284EA 182.33 USD
89160-284
FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences
Protein Reagents Protein Extraction Reagents
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

  • An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate.

Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an 'inhibitor stop' will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
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