5-Carboxyfluorescein 95%
Supplier: Thermo Scientific Chemicals
5-Carboxyfluorescein 95%
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5-Carboxyfluorescein Isomer_single 97%
Supplier: Thermo Scientific Chemicals
5-Carboxyfluorescein Isomer_single 97%
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5-Carboxyfluorescein
Supplier: Bachem Americas
Sequence: 5-Carboxy-fluorescein
Synonym(s): 5-FAM#4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophthalic acid
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5-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator
Supplier: Biotium
5-Carboxyfluorescein has a pKa of 6.5 and can be used as a pH sensor. The excitation spectrum of the dye responds to pH similarly to that of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
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5-Carboxyfluorescein ≥95%
Supplier: AAT BIOQUEST INC
5-FAM is the purified single isomer of carboxyfluorescein.
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5-Carboxyfluorescein ≥99% (by HPLC)
Supplier: Adipogen
The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
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5-Carboxyfluorescein
Supplier: Anaspec Inc
5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.