Supplier: Thermo Scientific Chemicals

MDL: MFCD00004116 Beilstein: 2206394 Slightly soluble in water

Supplier: Thermo Scientific Chemicals

2,3-Diaminonaphthalene 97%

Supplier: ABCAM INC CA

2,3-Diaminonaphthalene

Supplier: BeanTown Chemical

CAS: 771-97-1; EC No: 212-241-0; MDL No: MFCD00004116 Powder; Molecular Formula: C10H10N2; MW: 158.20

Supplier: Ambeed

2,3-Diaminonaphthalene 97%

Supplier: Biotium

reacts with nitrosonium, which is formed from NO, to form the fluorescent dye 1 H-naphthotriazole. Using this method, 10nM to 10uM of nitrite (NO2-) can be detected. The detection is compatible with 96-well format.

Supplier: AAT Bioquest

The role of nitric oxide (NO) as a putative mediator of neuronal death can be understood best if NO is detected directly.

Supplier: Adipogen

The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.