EZ-Tn5™ Transposase, Bacterial Mutagenesis, Biosearch Technologies

Supplier: Biosearch Technologies
TNP92110
75927-976EA 847.64 USD
75927-976
EZ-Tn5™ Transposase, Bacterial Mutagenesis, Biosearch Technologies
Nucleic Acid Reagents Nucleic Acid Modification and Labelling
Customize your in vitro or in vivo transposomics.

  • Create custom transposomes for in vitro or in vivo transposomics
  • Easily genereate libraries of mutants for metagenomics, functional genomics and strain engineering applications
  • Integrate your selectable marker, promoter or replication origin into plasmid or genomic DNA
  • Insert up to 12 kb of sequence - large enough for a biosynthetic pathway or operon

EZ-Tn5™ Transposase is a hyperactive form of Tn5 transposase

A typical EZ-Tn5 transposition reaction requires four components: (1) the EZ-Tn5 Transposase; (2) an EZ-Tn5 Transposon; (3) a target DNA; and (4) the presence of Mg2+. The highly random insertion of an EZ-Tn5 Transposon into the target DNA proceeds by a cut-and-paste mechanism, catalyzed by the EZ-Tn5 Transposase, and results in a 9-bp duplication of target DNA sequence immediately adjacent to both ends of the Transposon.

This product is covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).

The highly purified, single-subunit enzyme can be used to randomly insert (transpose or "hop") any EZ-Tn5 Transposon into any target DNA in vitro with an efficiency up to >106 insertion clones per standard reaction. When incubated with an EZ-Tn5 Transposon in the absence of Mg2+, a stable EZ-Tn5 Transposome™ complex is formed. The Transposome is so stable that it can be electroporated into living cells. Once in the cell, the Transposome is activated by intracellular Mg2+ and the EZ-Tn5 Transposon component is randomly inserted into the host's genomic DNA. The three-dimensional structure of a Transposome complex has been elucidated.

Información de suministro: Each kit contains 10 units of EZ-Tn5 Tansposase, which is enough enzyme for 10 standard reactions.

Please note: Lucigen and Illumina require a Transposome™ License Acknowledgement is signed prior to processing orders for EZ-Tn5™ Transposase. Please complete the form (attached to this product page) before submitting your online order, and email the completed signed form to custserv@lucigen.com.

Precaución: For research use only. Not for human or diagnostic use.
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