Practical UHPLC: Selectivity and rapid method development, method translations and instrument transfers
This discussion explores UHPLC method development approaches from simple to complex, and how different variables influence selectivity for the chromatographer. With an understanding of selectivity, and using a variety of chromatographic data, stationary phase design principles are explored including how different chemistries can help the analyst. This is illustrated using a related substances method development activity. The principles of method translation (HPLC to UHPLC and vice-versa) is demonstrated. Transferring a gradient UHPLC method between 4 different instruments, (including 3 different UHPLC instrument vendors) is also shown with hints and tips for success. Finally, high resolution UHPLC separations are shown.
Presented By : Alan McKeown
As an experienced chromatographer in his own right, Alan has been invited and delivered chromatography technical seminars in Europe, Asia, North America and South America at conferences, customer sites and organised events.
Alan has published various peer-reviewed articles and written different book chapters on separation science, chromatography and genotoxic impurities.
Alan is a Fellow of the Royal Society of Chemistry and a Chartered Chemist.