Cloning & DNA Assembly

Built on over 40 years of experience, New England Biolabs provides you with the quality reagents and technical support that you need to take your cloning experiments to the next level.  With reagents for each step in the cloning workflow, NEB products deliver quality and performance so that you can Clone with ConfidenceTM.

Synthetic biology is a recent expansion of the biotechnology field, in which genes are viewed as parts with the goal of assembling them in novel ways to create new functionalities. NEB has developed multiple products to help with these DNA assembly projects.

Product selection

Cloning Competent E.coli

Ensure successful transformations with NEB competent E. coli for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value.

NEBuilder® HiFi DNA Assembly

NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches.

Restriction Enzymes from NEB

With >285 restriction enzymes that are 100% active in a single buffer, CutSmart, it is much simpler to set up your double digest reactions.

Q5 Site-Directed Mutagenesis Kit

The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.