Gene Editing Technology, Tools, and Resources
Gene Editing is the application area concerned with the intentional alteration of the DNA sequence of a plasmid, cell or organism. Gene editing encompasses numerous methods including mutagenesis, restriction cloning, synthetic biology and CRISPR/Cas9, among others.
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Superior speed and inhibitor tolerance including high fidelity, extreme speed, and more!
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Ingenio® Electroporation Kits and Solution provide a universal, high efficiency, low toxicity solution for electroporation of DNA or siRNA into hard to transfect cell types
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What Is Gene Editing?
Gene Editing, or genome editing, is concerned with the intentional alteration of the DNA sequence of a plasmid, cell, or organism. Gene editing technology includes numerous methods including mutagenesis, restriction cloning, synthetic biology, and CRISPR/Cas9, among others.
Your lab can achieve profound breakthroughs in genomics with the right gene editing tools. Whether you are looking for mutagenesis kits to introduce point mutations or conducting mammalian gene editing, Avantor carries gene editing products and trusted solutions for every workflow.
How Many Gene Editing Techniques Are There?
There are currently four main gene editing techniques used by scientists:
- Restriction enzymes: DNA sequences are cut at a specific site using restriction enzymes. These enzymes only recognize certain patterns of nucleotide sequences which makes them somewhat limiting.
- Zinc finger nucleases (ZFNs): ZFNs provide a more precise gene editing technique than restriction enzymes. A zinc finger is fused on an engineered nuclease (Fokl) and recognizes a 3-base pair site on DNA. ZFNs can be combined to recognize larger sequences.
- TALENs: Similar to ZFNs, TALENs cut DNA using the Fokl nuclease, however, the binding domains differ. Target capabilities are greater due to the use of transcription activator-like effectors (TALEs).
- CRISPR/Cas9: Two components make up the CRISPR/cas9 gene editing tool: a guide RNA and a Cas9 nuclease. This technique offers greater customization to scientists.
Different gene editing technology may be chosen depending on the type of application and the goal of the edit. Gene editing may occur for any of the following purposes:
- Delete nucleotides (gene knockout).
- Add nucleotides (gene knock-in).
- Edit nucleotides to create a mutation.
What Are the Applications of Gene Editing Technology?
Since the structure of DNA was discovered, scientists have advanced our understanding of genomics considerably. Gene editing accomplishes many sought-after goals, and its applications continue to expand. The most prominent applications include those in the following areas:
Within the field of medicine, gene editing technology is used for drug discovery, to model disease, for gene therapy, or as a diagnostic tool.
Gene editing can be used to improve the food supply in numerous ways:
- Increase sustainability
- Improve nutritional value
- Create disease resistance
- Increase availability
Gene editing can also be useful in the area of identifying and changing genetic traits that can spread to entire populations. The potential exists to prevent disease and control pests when genomes of entire animal populations are edited.
Genome editing is also being used to develop and advance sustainable biofuels to replace harmful fossil fuels.
Find Products and Solutions for Your Gene Editing Applications
You can get all your gene editing products for every stage of the gene editing process from a single source. Avantor offers a comprehensive range of solutions optimized to fit your processes. We can help you obtain the results you need for every genome editing application.