Gene Editing Technology, Tools and Resources

Gene editing is the application area concerned with the intentional alteration of the DNA sequence of a plasmid, cell or organism. It encompasses numerous methods, including mutagenesis, restriction cloning, synthetic biology and CRISPR/Cas9, among others. Avantor^® carries all the technology, tools and resources you need for every gene editing application.

Find Products and Solutions for Your Gene Editing Applications

You can get all your gene editing products for every stage of the gene editing process from a single source. Avantor offers a comprehensive range of solutions optimized to fit your processes. We can help you obtain the results you need for every genome editing application. Learn more.

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Literature

Publications

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Questions about our gene editing technology, tools or resources?

Featured Suppliers for Gene Editing

Quantabio – advanced DNA and RNA Amplification Reagents

Quantabio amplification reagents for the most demanding molecular testing applications in applied, translational and life science research.

Life Science Solutions from Analytik Jena

Analytik Jena is a leading provider of life science products, including BioImaging, PCR, and Real-Time PCR systems for academic, pharmaceutical and biotechnology applications.

Construction

Obtain proven DNA construction products to effectively assemble genes or fragments of DNA sequences together.

Ligases

DNA and RNA ligases for cloning, mutagenesis and NGS

Mutagenesis kits

Kits to perform single and multiple site-directed mutagenesis

DNA gel stains

Stain solutions to detect DNA and RNA in electrophoresis gels

Restriction enzymes

Broad selection of restriction enzymes to cleave DNA molecules at specific sites

Synthetic biology kits

Kits to insert DNA on vectors

CRISPR/Cas9

Enzymes and libraries for CRISPR/Cas9

Vectors

Vectors for cloning and expression

Modifying enzymes

Enzymes to modify nucleic acid molecules

Transformation & Amplification

Our wide range of high-quality products includes everything your lab needs for successful transformation and amplification processes.

Competent cells

We offer a large variety of competent cells for cloning and protein work.

Dehydrated microbiology media

Cultivate and isolate microorganisms for research purposes. Fast-dissolving powders come premixed for quick preparation using neutral liquids.

Microbiology additives and supplements

Microbiology additives and supplements enhance the growth effectiveness in base media and allow researchers to customize the media accordingly to their needs.

Agars for microbiology

Agar powder for bacteriological applications

Prepared microbiology media

Plated or contained in tubes to guarantee that the correct nutrient mixtures are present for specific cell types.

Cloning cylinders

Glass cylinders to clone a single cell or group of cells

HiFi polymerases

Find a selection of Hi Fidelity DNA Polymerases

Electroporators

View all the best-in-class Electroporators we offer

Electroporation cuvettes

A full line of Electroporation Cuvettes to meet your needs.

Electroporation buffers

Increase transformation and transposition efficiencies with a high-quality Electroporation Buffer from Avantor

TAQ polymerases

TAQ DNA Polymerases for general PCR

End point thermal cyclers

See all the PCR Cyclers we offer.

PCR workstations

PCR workstation enclosures provide the contamination-free environment required for the most sensitive DNA segment detection.

PCR master mixes

Master Mixes provide everything needed to perform PCR. Just add template and primers and start amplifying

dNTPs

View our wide selection of high-purity Nucleotides.

PCR plates

Choose from our extensive variety of PCR plates

Microplate sealing films & foils

Broad range of microplate sealing films and foils to reduce evaporation during reactions

PCR tubes

Discover our large selection of high-quality PCR tubes.

Filter tips

Filters will block aerosol contaminants to protect both the equipment and sample.

Extraction & Purification

Ease the extraction and purification process with systems and kits designed for efficient operation and reliable end results.

PCR cleanup kits

Removal of unincorporated dNTPs, salts and enzymes after any PCR reaction

Automated nucleic acid purification systems

Get the automated nucleic acid purification systems you need for your lab.

Nucleic acid storage buffers

Buffers and systems for nucleic acid stability during sample storage/transport

Plasmid purification systems and kits

See our full offering of nucleic acid purification kits and Reagents.

Sonicators

Ultrasonic cell disrupters are effective for DNA/RNA shearing, homogenization, cell lysis, nanoparticle dispersion, protein extraction, and general sample preparation.

Surface decontamination

Reagents to remove DNase/RNase/DNA

Spectrophotometers

View our offering of UV/Visible spectrophotometers.

Construct Analysis

Perform accurate construct analysis using trusted gene editing products.

Agarose

See our entire agarose and acrylamide offering.

DNA MW markers & ladders

Molecular weight markers are ideal for verification of PCR products and for size analysis of digested DNA.

Gel imagers

Gel documentation systems

Transilluminators

We offer a large variety of transilluminators to meet your needs.

Horizontal gel electrophoresis systems

View our wide selection of horizontal gel electrophoresis systems.

Electrophoresis sequencers

Find here electrophoresis sequencing systems that allow quick assembly.

Spectrophotometers

Find here electrophoresis sequencing systems that allow quick assembly.

Power supplies

Select the power supply you need for your electrophoresis and blotting applications.

UV crosslinkers

Find the most popular UV crosslinkers.

Hybridization equipment

Choose from our selection of hybridization ovens with rapid heat-up capabilities and precise temperature control.

What Is Gene Editing?

Gene Editing, or genome editing, is concerned with the intentional alteration of the DNA sequence of a plasmid, cell, or organism. Gene editing technology includes numerous methods including mutagenesis, restriction cloning, synthetic biology and CRISPR/Cas9, among others.

Your lab can achieve profound breakthroughs in genomics with the right gene editing tools. Whether you are looking for mutagenesis kits to introduce point mutations or conducting mammalian gene editing, Avantor carries gene editing products and trusted solutions for every workflow.

How Many Gene Editing Techniques Are There?

There are currently four main gene editing techniques used by scientists:

Restriction Enzymes
DNA sequences are cut at a specific site using restriction enzymes. These enzymes only recognize certain patterns of nucleotide sequences which makes them somewhat limiting.

Zinc Finger Nucleases (ZFNs)
ZFNs provide a more precise gene editing technique than restriction enzymes. A zinc finger is fused on an engineered nuclease (Fokl) and recognizes a 3-base pair site on DNA. ZFNs can be combined to recognize larger sequences.

TALENs
Similar to ZFNs, TALENs cut DNA using the Fokl nuclease, however, the binding domains differ. Target capabilities are greater due to the use of transcription activator-like effectors (TALEs).

CRISPR/Cas9
Two components make up the CRISPR/cas9 gene editing tool: a guide RNA and a Cas9 nuclease. This technique offers greater customization to scientists.

Different gene editing technology may be chosen depending on the type of application and the goal of the edit. Gene editing may occur for any of the following purposes:

Delete nucleotides (gene knockout).
Add nucleotides (gene knock-in).
Edit nucleotides to create a mutation

What Are the Applications of Gene Editing Technology?

Since the structure of DNA was discovered, scientists have advanced our understanding of genomics considerably. Gene editing accomplishes many sought-after goals, and its applications continue to expand. The most prominent applications include those in the following areas:

Medicine
Within the field of medicine, gene editing technology is used for drug discovery, to model disease, for gene therapy, or as a diagnostic tool.

Agriculture
Gene editing can be used to improve the food supply in numerous ways:

Increase sustainability
Improve nutritional value
Create disease resistance
Increase availability

Population Health
Gene editing can also be useful in the area of identifying and changing genetic traits that can spread to entire populations. The potential exists to prevent disease and control pests when genomes of entire animal populations are edited.

Biofuels
Genome editing is also being used to develop and advance sustainable biofuels to replace harmful fossil fuels.

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